Instructions for use Title Structural Elucidation of Dextran Degradation Mechanism by Streptococcus mutans
نویسندگان
چکیده
Right This research was originally published in Journal of Biological Chemistry. Nobuhiro Suzuki; YoungMin Kim; Zui Fujimoto; Mitsuru Momma; Masayuki Okuyama; Haruhide Mori; Kazumi Funane; Atsuo Kimura. Structural Elucidation of Dextran Degradation Mechanism by Streptococcus mutans Dextranase Belonging to Glycoside Hydrolase Family 66. Journal of Biological Chemistry. 2012; 287:19916-19926. © the American Society for Biochemistry and Molecular Biology. Type article (author version)
منابع مشابه
Mechanism of adherence of Streptococcus mutans to smooth surfaces. I. Roles of insoluble dextran-levan synthetase enzymes and cell wall polysaccharide antigen in plaque formation.
The mechanism of adherence of Streptococcus mutans to smooth glass surfaces has been studied. The results with both viable and heat-killed cells showed that the process required (i) the synthesis of a water-insoluble dextran-levan polymer by cell-bound enzymes and (ii) the participation of a binding site on the surface of the S. mutans cell. Synthesis of the polymer from sucrose in the presence...
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Glucose-grown washed cells of streptococci similar to Streptococcus mutans, which contain cell-bound dextransucrase, have been observed to agglutinate upon the addition of high molecular weight dextran. Low molecular weight dextran or unrelated polysaccharides were ineffective. Agglutination also occurred upon addition of sucrose, which can be converted into dextran, but not with other mono- an...
متن کاملCrystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase.
Dextran glucosidase from Streptococcus mutans is an exo-hydrolase that acts on the nonreducing terminal alpha-1,6-glucosidic linkage of oligosaccharides and dextran with a high degree of transglucosylation. Based on amino-acid sequence similarity, this enzyme is classified into glycoside hydrolase family 13. Recombinant dextran glucosidase was purified and crystallized by the hanging-drop vapou...
متن کاملAdherence of Streptococcus mutans to dextran synthesized in the presence of extracellular dextransucrase.
Live or heat-killed cells of Streptococcus mutans specifically adhere to dextran previously synthesized on glass surfaces by the action of extracellular dextransucrase.
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Growth of Streptococcus mutans 6715 in a medium containing trace amounts of sucrose or dextran promotes cell-associated glucosyl transferase activity and increases the dextran-binding capacity of the organisms.
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